Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence

Author: Bignami, A.; Eng, L.F.; Dahl, D.; Uyeda, C.T.

Description: The glial fibrillary acidic (GFA) protein, a brain specific protein extracted from severely gliosed human tissue, is not species specific; cross-reaction occurs between anti-human GFA protein antibodies and brain extracts of rabbit, guinea pig, rat and dog. Using anti-GFA protein antiserum, astrocytes are selectively stained with the indirect immunofluorescence technique in both normal and pathological (gliosed) brain tissue.

Subject headings: Animals; Antibodies; Antibody Formation; Brain/pathology; Brain Chemistry; Cerebellum/immunology; Cross Reactions; Dementia/pathology; Dogs/immunology; Fluorescent Antibody Technique; Gliosis/pathology; Guinea Pigs/immunology; Humans; Immune Sera; Male; Medulla Oblongata/immunology; Middle Aged; Nerve Tissue Proteins; Neuroglia; Optic Nerve/immunology; Rabbits/immunology; Rats/immunology

Publication year: 1972

Journal or book title: Brain Research

Volume: 43

Issue: 2

Pages: 429-435

Find the full text : https://www.sciencedirect.com/science/article/pii/0006899372903988

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Type: Journal Article

Serial number: 113