Author: Knezevic, Petar; Voet, Marleen; Lavigne, Rob
Description: Pf1-like bacteriophages (family Inoviridae) of Pseudomonas aeruginosa can contribute to bacterial short term evolution and virulence. Here we examine Pf1-like (pro)phage diversity and prevalence among different P. aeruginosa isolates. Pf1-like prophages in sequenced genomes of P. aeruginosa were analyzed and grouped into four clades: Pf4, Pf5, Pf7 and Pf-LES. P. aeruginosa strains (n=241) were screened for the presence of universal (primers PfUa and PfUb) and specific Pf1-like genetic elements (Pf1, Pf4 and Pf5). More than half of the strains contained at least one Pf1-like genetic element (60%); universal elements were detected in 56% of the strains, Pf4 in 22%, Pf1 in 18% and Pf5 in 7%. Infectivity experiments confirmed that strains yielding PCR products with either universal or Pf4 specific primers can release infective virions. Based on the high prevalence of Pf1-like (pro)phages, it is necessary to further examine their involvement in P. aeruginosa virulence.
Subject headings: Cluster Analysis; DNA Primers; DNA, Bacterial; Genetic Variation; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; Prevalence, Prophages; Pseudomonas Phages; Pseudomonas aeruginosa; Sequence Analysis; DNA; Sequence Homology; Pf1-like phage; Prevalence; Pseudomonas aeruginosa prophage
Publication year: 2015
Journal or book title: Virology
Volume: 483
Pages: 64-71
Find the full text: https://www.sciencedirect.com/science/article/pii/S0042682215002056
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Serial number: 3324