Bacterial protein synthesis: the effects of antibiotics

Author: Cundliffe, E.; McQuillen, K.

Description: Puromycin induced the release of nascent peptides from ribosomes. We have studied this effect in vivo using protoplasts of Bacillus megaterium. Chloramphenicol, erythromycin and sparsomycin inhibited the reaction, but chlortetracycline, bottromycin and pactamycin did not.

In the presence of chlortetracycline, both chloramphenicol and sparsomycin completely inhibited the puromycin reaction, whereas erythromycin allowed release of much of the nascent peptide.

We suggest that chloramphenicol and sparsomycin inhibit the peptidyl transferase reaction, and that erythromycin may inhibit the translocation reaction(s).

Subject headings: Amino Acids/metabolism; Anti-Bacterial Agents/pharmacology; Bacillus megaterium/drug effects/metabolism; Bacterial Proteins/biosynthesis; Carbon Isotopes; Centrifugation, Density Gradient; Chloramphenicol/pharmacology; Chlortetracycline/pharmacology; Deoxyribonucleases/pharmacology; Erythromycin/pharmacology; Peptides/metabolism; Phosphates/metabolism; Phosphorus Isotopes; Protoplasts/metabolism; Puromycin/pharmacology; Ribonucleases/pharmacology; Ribosomes/drug effects/metabolism

Publication year: 1967

Journal or book title: Journal of Molecular Biology

Volume: 30

Issue: 1

Pages: 137-146

Find the full text : http://www.sciencedirect.com/science/article/pii/0022283667902495

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Type: Journal Article

Serial number: 531