Ghrelin and des-acyl ghrelin: two major forms of rat ghrelin peptide in gastrointestinal tissue

Author: Hosoda, H.; Kojima, M.; Matsuo, H.; Kangawa, K.

Description: Ghrelin, a novel peptide purified from stomach, is the endogenous ligand for the growth hormone secretagogue receptor and has potent growth hormone-releasing activity. The Ser3 residue of ghrelin is modified by n-octanoic acid, a modification necessary for hormonal activity. We established two ghrelin-specific radioimmunoassays; one recognizes the octanoyl-modified portion and another the C-terminal portion of ghrelin. Using these radioimmunoassay systems, we found that two major molecular forms exist-ghrelin and des-n-octanoyl ghrelin. While ghrelin activates growth-hormone secretagogue (GHS) receptor-expressing cells, the nonmodified des-n-octanyl form of ghrelin, designated as des-acyl ghrelin, does not. In addition to these findings, our radioimmunoassay systems also revealed high concentrations of ghrelin in the stomach and small intestine.

Subject headings: Amino Acid Sequence; Animals; Duodenum; Gastric Mucosa; Ghrelin; Immunoglobulins; Male; Molecular Sequence Data; Peptide Hormones; Peptides; Radioimmunoassay; Rats; Rats; Sprague-Dawley; Tissue Distribution

Publication year: 2000

Journal or book title: Biochemical and Biophysical Research Communications

Volume: 279

Issue: 3

Pages: 909-913

Find the full text: https://www.sciencedirect.com/science/article/abs/pii/S0006291X00940397

Find more like this one (cited by): https://scholar.google.com/scholar?cites=7006765947106339192&as_sdt=400005&sciodt=0,14&hl=en

Serial number: 3403

Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.