Author: Lomedico, P.T.; Saunders, G.F.
Description: Total nucleic acid extraction and selection of poly A-containing molecules yield preparative quantities of undegraded mRNA from adult and fetal pancreas. Using a stringent immunoassay, this mRNA is found to direct the synthesis of an immunoreactive insulin polypeptide in the wheat germ translation system. On sodium dodecyl sulfate polyacrylamide gels, this polypeptide (12,000-13,000 daltons) is larger than proinsulin (9,000 daltons).
Subject headings: Aging; Animals; Dogs; Fetus; Molecular Weight; Pancreas/growth & development/metabolism; Peptide Biosynthesis; Poly A/metabolism; Precipitin Tests; Proinsulin/biosynthesis/immunology; Protein Biosynthesis; RNA, Messenger/metabolism; Seeds/metabolism; Triticum/metabolism
Publication year: 1976
Journal or book title: Nucleic Acids Research
Volume: 3
Issue: 2
Pages: 381-391
Find the full text : https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.918.3804&rep=rep1&type=pdf
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Type: Journal Article
Serial number: 1282