Crystal structure of the nucleosome core particle at 2.8 A resolution

Author: Luger, K.; Mader, A.W.; Richmond, R.K.; Sargent, D.F.; Richmond, T.J.

Description: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it. Both histone/histone and histone/DNA interactions depend on the histone fold domains and additional, well ordered structure elements extending from this motif. Histone amino-terminal tails pass over and between the gyres of the DNA superhelix to contact neighbouring particles. The lack of uniformity between multiple histone/DNA-binding sites causes the DNA to deviate from ideal superhelix geometry.

Subject headings: Amino Acid Sequence; Base Composition; Crystallography, X-Ray; DNA/chemistry; DNA, Superhelical/chemistry; Histones/chemistry; Humans; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Nucleosomes/chemistry; Protein Binding; Protein Conformation; Protein Folding

Publication year: 1997

Journal or book title: Nature

Volume: 389

Issue: 6648

Pages: 251-260

Find the full text: https://www.strategian.com/fulltext/Luger1997.pdf

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Type: Journal Article

Serial number: 1989