A Modified Agar Plate Method for Detection of Strongyloides Stercoralis

Author: Kita, K.; Ohtomo, H.; Koga, K.; Kasuya, S.; Khamboonruang, C.; Sukhavat, K.; Ieda, M.; Takatsuka, N.

Description: The agar plate method is a new technique with high detection rates for coprological diagnosis of human stronglyoidiasis. This report details modifications of the technique and establishes a standardized procedure. We recommend that all plates should be carefully observed using a microscope because macroscopic observation can lead to false negative results. It is also advisable to pour formalin solution directly into microscopically positive dishes to collect worms by sedimentation. This procedure enables one to observe worms otherwise hidden. Sealing dishes with adhesive tape prevents larvae from crawling out of the dishes, eliminating any possibility in the reduction of detection rates, and greatly improves the safety conditions for the technician performing the procedure. We consider the agar plate method to be superior to the filter paper method in detecting Strongyloides, and we believe that it will eventually become the technique of choice.

Subject headings: Agar plate method; Coprological diagnosis; Macroscopic

Publication year: 1991

Journal or book title: The American Journal of Tropical Medicine and Hygiene

Volume: 45

Issue: 4

Pages: 518-521

Find the full text : https://europepmc.org/article/med/1951861

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Type: Journal Article

Serial number: 2161