Author: Forman, H.J.; Augusto, O.; Brigelius-Flohe, R.; Dennery, P.A.; Kalyanaraman, B.; Ischiropoulos, H.; Mann, G.E.; Radi, R.; Roberts, L.J. 2nd; Vina, J.; Davies, K.J.A.
Description: Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species.
Subject Headings: Animals; Antioxidants/metabolism; Fluorescent Dyes/chemistry; Free Radical Scavengers/chemistry; Free Radicals/analysis/chemistry; Humans; Lipid Peroxidation; Reactive Nitrogen Species/chemistry; Reactive Oxygen Species/chemistry; Terminology as Topic; Thiobarbituric Acid Reactive Substances; Antioxidant; F2-isoprostane; Fluorescent dye; Free radicals; Radical scavenging; Reactive nitrogen species; Reactive oxygen species; Thiobarbituric acid; alpha-Tocopherol
Subject headings:
Publication year: 2015
Journal or book title: Free Radical Biology & Medicine
Volume: 78
Issue:
Pages: 233-235
Find the full text : https://www.sciencedirect.com/science/article/pii/S0891584914010053
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Type: Journal Article
Serial number: 2207