Author: Ziegler, K.; Unanue, E.R.
Description: The mechanism of macrophage-antigen handling was studied using a system that involves the quantitation of the antigen-specific binding of Listeria monocytogenes-immune T cells to macrophages. Specific T cells did not bind to native antigen. Because the specific binding of T cells to macrophages could be measured during a short (5- to 15-min) interaction, it was possible to follow the temporal development of a T cell-binding substrate with increasing time of antigen-macrophage interaction. In contrast to the rapid (5-min) uptake of Listeria by macrophages, the development of T cell-binding ability required a 30- and 60-min period of antigen-macrophage interaction. During this processing period, Listeria organisms bound to the macrophage surface were ingested and partially catabolized. Unlike antigen uptake, antigen processing was a temperature-dependent and energy-requiring event. Although macrophages treated with paraformaldehyde before antigen processing did not develop T cell-binding activity, macrophages treated with paraformaldehyde after a 60-min antigen-processing period retained T cell-binding ability. The kinetics of antigen catabolism correlated with antigen processing, and inhibition of antigen catabolism was associated with a corresponding inhibition of antigen processing for T cell binding. Anti-Ia antibodies had no effect on Listeria uptake of catabolism. These results supply direct evidence for a macrophage-antigen processing event relevant to T cell recognition of antigen.
Subject Headings: Animals; Antigens; Binding Sites; Female; H-2 Antigens; Kinetics; Listeria monocytogenes/immunology; Macrophages/immunology/metabolism; Male; Mice; Mice, Inbred A; T-Lymphocytes/immunology
Keywords: Identification of a macrophage antigen-processing event required for I-region-restricted antigen presentation to T lymphocytes
Publication year: 1981
Journal or book title: Journal of Immunology (Baltimore, Md. : 1950)
Volume: 127
Issue: 5
Pages: 1869-1875
Find the full text : https://www.jimmunol.org/content/127/5/1869.short
Find more like this one (cited by): https://scholar.google.com/scholar?cites=416758632222358647&as_sdt=1000005&sciodt=0,16&hl=en
Type: Journal Article
Serial number: 2553