Grapefruit juice and its furocoumarins inhibits autoinducer signaling and biofilm formation in bacteria

Author: Girennavar, B.; Cepeda, M.L.; Soni, K.A.; Vikram, A.; Jesudhasan, P.; Jayaprakasha, G.K.; Pillai, S.D.; Patil, B.S.

Description: Cell-to-cell communications in bacteria mediated by small diffusible molecules termed as autoinducers (AI) are known to influence gene expression and pathogenicity. Oligopeptides and N-acylhomoserine lactones (AHL) are major AI molecules involved in intra-specific communication in gram-positive and gram-negative bacteria respectively, whereas boronated-diester molecules (AI-2) are involved in inter-specific communication among both gram-positive and gram-negative bacteria. Naturally occurring furocoumarins from grapefruit showed >95% inhibition of AI-1 and AI-2 activities based on the Vibrio harveyi based autoinducer bioassay. Grapefruit juice and furocoumarins also inhibited biofilm formation by Escherichia coli O157:H7, Salmonella typhimurium and Pseudomonas aeruginosa. These results suggest that grapefruit juice and furocoumarins could serve as a source to develop bacterial intervention strategies targeting microbial cell signaling processes.

Subject Headings: Analysis of Variance; Beverages; Biofilms/growth & development; Biomass; Citrus paradisi; Colony Count, Microbial; Escherichia coli O157/drug effects/physiology; Food Contamination/prevention & control; Food Microbiology; Furocoumarins/isolation & purification/pharmacology; Gram-Negative Bacteria/drug effects/physiology; Gram-Positive Bacteria/drug effects/physiology; Pseudomonas aeruginosa/drug effects/physiology; Quorum Sensing; Salmonella typhimurium/drug effects/physiology; Signal Transduction

Publication year: 2008

Journal or book title: International Journal of Food Microbiology

Volume: 125

Issue: 2

Pages: 204-208

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Type: Journal Article

Serial number: 2798