Simultaneous determination of citrus limonoid aglycones and glucosides by high performance liquid chromatography

Author: Vikram, A.; Jayaprakasha, G.K.; Patil, B.S.

Description: High performance liquid chromatography (HPLC) method has been developed for simultaneous quantification of limonoid aglycones and glucosides on a reversed phase C18 column using a binary solvent system, coupled with diode array detector. Seven limonoids such as limonin, nomilin, isolimonic acid, ichangin, isoobacunoic acid, limonin 17-beta-D glucopyranoside and deacetyl nomilinic acid 17-beta-D glucopyranoside were separated and detected at 210 nm. Furthermore, limonoids were separated, identified and quantified in four varieties of citrus fruits and seeds using developed method. Limonin and limonin glucoside were found to be the predominant limonoid aglycone and glucoside, respectively, in all tested samples. The sensitivity of the method was found to be 0.25-0.50 microg for tested limonoids.

Subject Headings: Chromatography, High Pressure Liquid/methods; Citrus/chemistry; Glucosides/analysis; Limonins/analysis; Seeds/chemistry

Keywords: Simultaneous determination of citrus limonoid aglycones and glucosides by high performance liquid chromatography

Publication year: 2007

Journal or book title: Analytica Chimica Acta

Volume: 590

Issue: 2

Pages: 180-186

Find the full text : https://www.sciencedirect.com/science/article/pii/S0003267007005442

Find more like this one (cited by): https://scholar.google.com/scholar?cites=14301844997183677354&as_sdt=1000005&sciodt=0,16&hl=en

Type: Journal Article

Serial number: 2805