Author: Zengin, N.; Yuzbasioglu, D.; Unal, F.; Yilmaz, S.; Aksoy, H.
Description: In this study, the genotoxic effects of sodium benzoate (SB) and potassium benzoate (PB) were investigated in cultured human peripheral lymphocytes using chromosomal aberrations (CA), sister chromatid exchange (SCE), and micronuclei (MN). The level of nuclear DNA damage of SB and PB were also evaluated using the comet assay. The lymphocytes were incubated with different concentrations of SB (6.25, 12.5, 25, 50, and 100 mug/ml) and PB (62.5, 125, 250, 500, and 1000 mug/ml). A significant increase was observed in CA, SCE, and MN, in almost all treatments compared to negative controls. SB and PB significantly decreased the mitotic index (MI) in all the treatments, compared to the negative controls. However, neither of the additives affected the replication index (RI). Although SB significantly increased DNA damage, PB did not cause a significant increase in DNA damage. The present results indicate that SB and PB are clastogenic, mutagenic and cytotoxic to human lymphocytes in vitro.
Subject headings: Benzoates/toxicity; Dose-Response Relationship, Drug; Food Preservatives/toxicity; Humans; Lymphocytes/drug effects; Mutagenicity Tests; Sodium Benzoate/toxicity
Keywords: The evaluation of the genotoxicity of two food preservatives: sodium benzoate and potassium benzoate
Publication year: 2011
Journal or book title: Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Volume: 49
Issue: 4
Pages: 763-769
Find the full text : https://www.sciencedirect.com/science/article/pii/S0278691510006988
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Type: Journal Article
Serial number: 2908