Author: Le Pecq, J.B.; Paoletti, C.
Description: Previous studies have shown that a dye, ethidium bromide, markedly increases its fluorescence when it binds to double-stranded DNA or double-stranded regions of RNA. It is shown that, in the given conditions, a linear relationship between fluorescence and nucleic acid concentration is obtained. Thus, a new spectrofluorometric method for determining DNA and RNA using this principle is described. This method is specific for nucleic acids, can be used over a wide range of salt concentrations, and has a good sensitivity (0.01 ug/ml of DNA). No biological compounds able to interfere have been found. An adaptation of this method for determining both DNA and RNA in a mixture is also described.
Subject Headings: Adenine Nucleotides; Animals; Blood Proteins; Cattle; Chondroitin; Cytosine Nucleotides; DNA/analysis; Deoxyribonucleases; Fluorometry; Guanine Nucleotides; Heparin; Hyaluronic Acid; Liver; Nucleotides; Phenanthrenes; RNA/analysis; Rats; Ribonucleases; Spectrophotometry; Thymus Gland
Publication year: 1966
Journal or book title: Analytical Biochemistry
Volume: 17
Issue: 1
Pages: 100-107
Find the full text : https://www.sciencedirect.com/science/article/pii/0003269766900121
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Type: Journal Article
Serial number: 2769