Author: Witze, Eric S.; Old, William M.; Resing, Katheryn A.; Ahn, Natalie G.
Description: Post-translational modifications of proteins control many biological processes, and examining their diversity is critical for understanding mechanisms of cell regulation. Mass spectrometry is a fundamental tool for detecting and mapping covalent modifications and quantifying their changes. Modern approaches have made large-scale experiments possible, screening complex mixtures of proteins for alterations in chemical modifications. By profiling protein chemistries, biologists can gain deeper insight into biological control. The aim of this review is introduce biologists to current strategies in mass spectrometry-based proteomics that are used to characterize protein post-translational modifications, noting strengths and shortcomings of various approaches.
Subject headings: Acetylation; Cysteine; Databases; Protein; Isotope Labeling; Mass Spectrometry; Oxidation-Reduction; Phosphopeptides; Phosphorylation; Protein Interaction Mapping; Protein Processing; Post-Translational; Tandem Mass Spectrometry; Ubiquitin
Publication year: 2007
Journal or book title: Nature Methods
Volume: 4
Issue: 10
Pages: 798-806
Find the full text: http://bio.ijs.si/~krizaj/group/Struktura%20proteinov%202012/Seminarji-osnovna%20literatura/8.Witze2007.pdf
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Serial number: 3966