Citrus flavonoid represses Salmonella pathogenicity island 1 and motility in S. Typhimurium LT2

Author: Vikram, A.; Jesudhasan, P.R.; Jayaprakasha, G.K.; Pillai, S.D.; Jayaraman, A.; Patil, B.S. Description: Salmonellosis is one of the leading health problems worldwide. With the rise of drug resistance strains, it has become imperative to identify alternative strategies to counter bacterial infection. Natural products were used historically to identify novel compounds with various bioactivities. Citrus species is a rich source of flavonoids. Naringenin, a flavonone, is present predominantly in grapefruit. Previously we have demonstrated that naringenin is potent inhibitor of cell-cell signaling. The current study was undertaken to understand the…

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Veterinary use and antibiotic resistance

Author: Teuber, M. Description: Globally, an estimated 50% of all antimicrobials serve veterinary purposes. Bacteria that inevitably develop antibiotic resistance in animals comprise food-borne pathogens, opportunistic pathogens and commensal bacteria. The same antibiotic resistance genes and gene transfer mechanisms can be found in the microfloras of animals and humans. Direct contact, food and water link animal and human habitats. The accumulation of resistant bacteria by the use of antibiotics in agriculture and veterinary medicine and the spread of such bacteria via agriculture and direct contamination are documented. Subject headings: Agriculture;…

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Stress responses as determinants of antimicrobial resistance in Gram-negative bacteria

Author: Poole, K. Description: Bacteria encounter a myriad of potentially growth-compromising conditions in nature and in hosts of pathogenic bacteria. These ‘stresses’ typically elicit protective and/or adaptive responses that serve to enhance bacterial survivability. Because they impact upon many of the same cellular components and processes that are targeted by antimicrobials, adaptive stress responses can influence antimicrobial susceptibility. In targeting and interfering with key cellular processes, antimicrobials themselves are ‘stressors’ to which protective stress responses have also evolved. Cellular responses to nutrient limitation (nutrient stress), oxidative and nitrosative stress, cell…

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Long-term application of fresh and composted manure increase tetracycline resistance in the arable soil of eastern China

Author: Peng, S.; Wang, Y.; Zhou, B.; Lin, X. Description: The aim of this study was to compare the occurrence, abundance, and diversity of tetracycline resistance genes (tet) in agricultural soils after 6 years’ application of fresh or composted swine manure. Soil samples were collected from fresh or composted manure-treated farmland at three depths (0-5 cm, 5-10 cm, and 10-20 cm). Nine classes of tet genes [tetW, tetB(P), tetO, tetS, tetC, tetG, tetZ, tetL, and tetX] were detected; tetG, tetZ, tetL, and tetB(P) were predominant in the manure-treated soil. The…

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Cooperation and competition in pathogenic bacteria

Author: Griffin, A.S.; West, S.A.; Buckling, A. Description: Explaining altruistic cooperation is one of the greatest challenges for evolutionary biology. One solution to this problem is if costly cooperative behaviours are directed towards relatives. This idea of kin selection has been hugely influential and applied widely from microorganisms to vertebrates. However, a problem arises if there is local competition for resources, because this leads to competition between relatives, reducing selection for cooperation. Here we use an experimental evolution approach to test the effect of the scale of competition, and how…

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Detection of hepatitis E virus and other livestock-related pathogens in Iowa streams

Author: Givens, C.E.; Kolpin, D.W.; Borchardt, M.A.; Duris, J.W.; Moorman, T.B.; Spencer, S.K. Description: Manure application is a source of pathogens to the environment. Through overland runoff and tile drainage, zoonotic pathogens can contaminate surface water and streambed sediment and could affect both wildlife and human health. This study examined the environmental occurrence of gene markers for livestock-related bacterial, protozoan, and viral pathogens and antibiotic resistance in surface waters within the South Fork Iowa River basin before and after periods of swine manure application on agricultural land. Increased concentrations of…

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Virulence factors in brucellosis: implications for aetiopathogenesis and treatment

Author: Fugier, E.; Pappas, G.; Gorvel, J.-P. Description: Brucella species are responsible for the global zoonotic disease brucellosis. These intracellular pathogens express a set of factors – including lipopolysaccharides, virulence regulator proteins and phosphatidylcholine – to ensure their full virulence. Some virulence factors are essential for invasion of the host cell, whereas others are crucial to avoid elimination by the host. They allow Brucella spp. to survive and proliferate within its replicative vacuole and enable the bacteria to escape detection by the host immune system. Several strategies have been used…

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Microbial ecology, bacterial pathogens, and antibiotic resistant genes in swine manure wastewater as influenced by three swine management systems

Author: Brooks, J.P.; Adeli, A.; McLaughlin, M.R. Description: The environmental influence of farm management in concentrated animal feeding operations (CAFO) can yield vast changes to the microbial biota and ecological structure of both the pig and waste manure lagoon wastewater. While some of these changes may not be negative, it is possible that CAFOs can enrich antibiotic resistant bacteria or pathogens based on farm type, thereby influencing the impact imparted by the land application of its respective wastewater. The purpose of this study was to measure the microbial constituents of…

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Incidence, contributing factors, and control of bacterial pathogens in produce

**top choice** Author: Brackett, R.E. Description: The importance of bacterial pathogens in the transmission of foodborne illness has become apparent in recent years. Several large, well-publicized outbreaks of foodborne illness have been linked to cantaloupe, tomatoes, lettuce, alfalfa sprouts, and both apple and orange juices. In addition, numerous other smaller scale outbreaks linked to these and other commodities have also been reported. Although contributing factors have not been determined in all cases, several notable causes have been proposed. In particular, cross contamination with fecal matter of both domestic as well…

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Not in Man’s Image: Lesbians and the Cultural Oppression of Body Image

Author: Dworkin, S.H. Description: Explores how the cultural oppression of “looksism,” the societal belief in one ideal body type proposed by S. H. Dworkin and B. A. Kerr, has affected lesbians. The issues of socialization of body image for women and lesbians and fat politics are discussed. It is considered crucial when working with a lesbian, or any woman with body image problems, that therapists be clear about their own issues, beliefs, and values regarding weight. It is concluded that the lesbian feminist community is beginning to attack the patriarchal…

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