Ecology of Borrelia burgdorferi sensu lato in Europe: transmission dynamics in multi-host systems, influence of molecular processes and effects of climate change

Author: Mannelli, A.; Bertolotti, L.; Gern, L.; Gray, J. Description: The analysis of different multi-host systems suggests that even hosts that are not capable of transmitting Borrelia burgdorferi sensu lato (s.l.) to the tick vector, Ixodes ricinus, or that are secondary reservoirs for these agents contribute to the intensity of transmission and to the overall risk of Lyme borreliosis, through the process of vector augmentation and pathogen amplification. On the other hand, above certain threshold densities, or in the presence of competition with primary reservoir hosts or low attachment rate…

See more and a link to full text

Neuroscience, molecular biology, and the childhood roots of health disparities: Building a new framework for health promotion and disease prevention

Shonkoff, J. P., Boyce, W. T., & McEwen, B. S. (2009) JAMA, 301(21), 2252-2259 A scientific consensus is emerging that the origins of adult disease are often found among developmental and biological disruptions occurring during the early years of life. These early experiences can affect adult health in 2 ways—either by cumulative damage over time or by the biological embedding of adversities during sensitive developmental periods. In both cases, there can be a lag of many years, even decades, before early adverse experiences are expressed in the form of disease….

See more and a link to full text

Trypanosoma cruzi-induced molecular mimicry and Chagas’ disease

Author: Girones, N.; Cuervo, H.; Fresno, M. Description: Chagas’ disease, caused by Trypanosoma cruzi, has been considered a paradigm of infection-induced autoimmune disease. Thus, the scarcity of parasites in the chronic phase of the disease contrasts with the severe cardiac pathology observed in approximately 30% of chronic patients and suggested a role for autoimmunity as the origin of the pathology. Antigen-specific and antigen-non-specific mechanisms have been described by which T. cruzi infection might activate T and B cells, leading to autoimmunity. Among the first mechanisms, molecular mimicry has been claimed…

See more and a link to full text

Scalable molecular dynamics with NAMD

Author: Phillips, J.C.; Braun, R.; Wang, W.; Gumbart, J.; Tajkhorshid, E.; Villa, E.; Chipot, C.; Skeel, R.D.; Kale, L.; Schulten, K. Description: NAMD is a parallel molecular dynamics code designed for high-performance simulation of large biomolecular systems. NAMD scales to hundreds of processors on high-end parallel platforms, as well as tens of processors on low-cost commodity clusters, and also runs on individual desktop and laptop computers. NAMD works with AMBER and CHARMM potential functions, parameters, and file formats. This article, directed to novices as well as experts, first introduces concepts…

See more and a link to full text

Stem cell therapy in Alzheimer’s disease: possible benefits and limiting drawbacks

Author: Alipour, Masoume; Nabavi, Seyed Massood; Arab, Leila; Vosough, Massoud; Pakdaman, Hossein; Ehsani, Ehsan; Shahpasand, Koorosh Description: Alzheimer’s disease (AD) is the sixth leading cause of death globally and the main reason for dementia in elderly people. AD is a long-term and progressive neurodegenerative disorder that steadily worsens memory and communicating skills eventually leads to a disabled person of performing simple daily tasks. Unfortunately, numerous clinical trials exploring new therapeutic drugs have encountered disappointing outcomes in terms of improved cognitive performance since they are not capable of halting or stimulating…

See more and a link to full text

Bacterial protein synthesis: the effects of antibiotics

Author: Cundliffe, E.; McQuillen, K. Description: Puromycin induced the release of nascent peptides from ribosomes. We have studied this effect in vivo using protoplasts of Bacillus megaterium. Chloramphenicol, erythromycin and sparsomycin inhibited the reaction, but chlortetracycline, bottromycin and pactamycin did not. In the presence of chlortetracycline, both chloramphenicol and sparsomycin completely inhibited the puromycin reaction, whereas erythromycin allowed release of much of the nascent peptide. We suggest that chloramphenicol and sparsomycin inhibit the peptidyl transferase reaction, and that erythromycin may inhibit the translocation reaction(s). Subject headings: Amino Acids/metabolism; Anti-Bacterial Agents/pharmacology;…

See more and a link to full text

Spectral studies on the denaturation of myoglobin

Author: Schechter, A.N.; Epstein, C.J. Description: Absorption and fluorescence spectroscopy and optical rotation measurements have been used to follow the denaturation of sperm whale skeletal muscle and horse heart myoglobins by urea and guanidine hydrochloride. The spectral properties of the metmyoglobin forms of each of these proteins change concomitantly during denaturation, and the transitions are compatible with a one-step, cooperative denaturation process. The apomyoglobins from sperm whale and horse undergo transitions at lower concentrations of denaturants than do their respective metmyoglobins, and the data are again compatible with a one-step…

See more and a link to full text

Enumeration of bacteriophages by double agar overlay plaque assay

Author: Kropinski, Andrew M.; Mazzocco, Amanda; Waddell, Thomas E.; Lingohr, Erika; Johnson, Roger P. Description: The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described. Subject headings: Agar; Bacteriophages; Viral Plaque Assay; Phage; Protocol Publication year: 2009 Journal or book title: Methods in Molecular Biology Volume: 501 Pages: 69–76 Find the full text: http://psulibrary.palawan.edu.ph/wtbooks/resources/pdf/910853.pdf#page=86 Find more like this one (cited by): https://scholar.google.com/scholar?cites=9432238468770859004&as_sdt=5,26&sciodt=0,26&hl=en Serial number: 3198

See more and a link to full text

Enzyme kinetics: a modern approach

Author: Marangoni, Alejandro G. Description: Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses. Subject headings: Enzyme kinetics; Biochemistry cell and molecular biology Publication year: 2003 Pages: 248 Find the full text: https://www.wiley.com/en-us/Enzyme+Kinetics%3A+A+Modern+Approach-p-9780471461418 Find more like this one (cited by): https://scholar.google.com/scholar?cites=2617071681772723511&as_sdt=5,26&sciodt=0,26&hl=en Serial number: 3174

See more and a link to full text

Inositol 1,4,5-trisphosphate receptors are strongly expressed in the nervous system, pharynx, intestine, gonad and excretory cell of Caenorhabditis elegans and are encoded by a single gene (itr-1)

Author: Baylis, H.A.; Furuichi, T.; Yoshikawa, F.; Mikoshiba, K.; Sattelle, D.B. Description: Inositol 1,4,5-trisphosphate (InsP3) activates receptors (InsP3Rs) that mediate intracellular Ca(2+ )release, thereby modulating intracellular calcium signals and regulating important aspects of cellular physiology and gene expression. To further our understanding of InsP3Rs we have characterised InsP3Rs and the InsP3R gene, itr-1, from the model organism Caenorhabditis elegans. cDNAs encoding InsP3Rs were cloned enabling us to: (a) identify three putative transcription start sites that result in alternative mRNA 5′ ends: (b) detect alternative splicing at three sites and: (c)…

See more and a link to full text