Tag: DNA

DNA origami objects

A primer to scaffolded DNA origami

Student Database

Author: Castro, C.E.; Kilchherr, F.; Kim, D.-N.; Shiao, E.L.; Wauer, T.; Wortmann, P.; Bathe, M.; Dietz, H. Description: Molecular self-assembly with scaffolded DNA origami enables building custom-shaped nanometer-scale objects with molecular weights in the megadalton regime. Here we provide a practical guide for design and assembly of scaffolded DNA origami objects. We also introduce a computational tool for predicting the structure of DNA origami objects and provide information on the conditions under which DNA origami objects can be expected to maintain their structure. Subject Headings: Computer-Aided Design; DNA/chemistry/ultrastructure; DNA Primers/chemistry;…

See more and a link to full text

Dependence of the melting temperature of DNA on salt concentration

Student Database

Author: Schildkraut, C. Description: Data on the decrease of the DNA melting temperature Tm with the salt concentration M are reported and discussed. The electrostatic free energy change in the helix–coil transition, ΔFe is related to the potential, ψ, which represents the electrostatic repulsion between the phosphate charges; ψ is calculated as a function of M and of the distances between the charges of the two strands. The Debye-Hückel approximation is shown to overestimate ψ. It is suggested that the high local concentration of the counterions in the immediate vicinity…

See more and a link to full text
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.