Author: Strober, W.
Description: The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply mixed with dye and then visually examined to determine whether cells take up or exclude dye. In the protocol presented here, a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm.
Subject headings: Cell Survival/physiology; Cells/chemistry/metabolism; Cells, Cultured; Staining and Labeling/methods; Trypan Blue/analysis/metabolism
Publication year: 2001
Journal or book title: Current Protocols in Immunology
Volume:
Issue:
Pages: Appendix 3B
Find the full text :Â https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/0471142735.ima03bs21
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Type: Book Chapter
Serial number: 2165