Overexpression of GASA5 increases the sensitivity of Arabidopsis to heat stress

Author: Zhang, S.; Wang, X. Description: Basal thermotolerance is very important for plant growth and development when plants are subjected to heat stress. However, little is known about the functional mechanism of gibberellins (GAs) in the basal thermotolerance of plants. In the present work, we provide molecular evidence that a member of the gene family encoding the GA-stimulated Arabidopsis (GASA) peptides, namely GASA5, is involved in the regulation of seedling thermotolerance. The GASA5-overexpressing plants displayed a weak thermotolerance, with a faster cotyledon-yellowing rate, lower seedling-survival rate, and slower hypocotyl elongation,…

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Identification of two phenotypes of Arabidopsis thaliana under in vitro salt stress conditions

Author: Ruiz Carrasco, K.B.; Baroni Fornasiero, R.; Tassoni, A.; Bagni, N. Description: This study describes two phenotypes of Arabidopsis thaliana (ecotype Columbia) developed in vitro under salt stress (75 mM NaCl). The phenotypes 01 and 02 appeared visibly distinguishable by rosette morphology and competence to produce flowers. Phenotype 01, sensible to salt stress, accumulated high quantities of Na+, showed a slight reduction in dry mass, and high protein and chlorophyll contents. Moreover, its anatomy exhibited some xeromorphic traits. Phenotype 02, clearly salt tolerant, showed a morphology similar to control plants,…

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Physiological and biochemical changes of CBF3 transgenic oat in response to salinity stress

Author: Oraby, H.; Ahmad, R. Description: Salinity is a major abiotic constraint affecting oat productivity. Several physiological and biochemical traits have been found to be related to yield maintenance under salinity. The impact of introducing the Arabidopsis CBF3 gene controlled by the rd29A stress-inducible promoter in T(2) transgenic oat on salinity tolerance and associated physiological changes were studied. Compared with the non-transgenic control, transgenic T(2) plants exhibited greater growth and showed significant maintenance of leaf area, relative water content, chlorophyll content, photosynthetic and transpiration rates as well as increased levels…

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Regulation of Arabidopsis thaliana Ku genes at different developmental stages under heat stress

Author: Liu, P.F.; Wang, Y.K.; Chang, W.C.; Chang, H.Y.; Pan, R.L. Description: Ku, a heterodimeric protein consisting of 70- and 80-kDa subunits, is involved in many cellular processes, such as DNA replication, cell cycle regulation and heat shock response. Moreover, the expression of Arabidopsis thaliana Ku genes (AtKu) is modulated by certain plant hormones through several signal transduction pathways. This study investigated how AtKu are regulated by heat stress. AtKu expression in 3-week-old young seedlings was down-regulated by heat stress in a time-dependent manner, as examined using real-time quantitative PCR,…

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Comparative salt tolerance analysis between Arabidopsis thaliana and Thellungiella halophila, with special emphasis on K(+)/Na(+) selectivity and proline accumulation

Author: Ghars, M.A.; Parre, E.; Debez, A.; Bordenave, M.; Richard, L.; Leport, L.; Bouchereau, A.; Savoure, A.; Abdelly, C. Description: The eco-physiology of salt tolerance, with an emphasis on K(+) nutrition and proline accumulation, was investigated in the halophyte Thellungiella halophila and in both wild type and eskimo-1 mutant of the glycophyte Arabidopsis thaliana, which differ in their proline accumulation capacity. Plants cultivated in inert sand were challenged for 3 weeks with up to 500mM NaCl. Low salinity significantly decreased A. thaliana growth, whereas growth restriction was significant only at…

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Analysis of short-term changes in the Arabidopsis thaliana glycerolipidome in response to temperature and light

Author: Burgos, A.; Szymanski, J.; Seiwert, B.; Degenkolbe, T.; Hannah, M.A.; Giavalisco, P.; Willmitzer, L. Description: Although the influence of temperature, particularly cold, on lipid metabolism is well established, previous studies have focused on long-term responses and have largely ignored the influence of other interacting environmental factors. Here, we present a time-resolved analysis of the early responses of the glycerolipidome of Arabidopsis thaliana plants exposed to various temperatures (4, 21 and 32 degrees C) and light intensities (darkness, 75, 150 and 400 mumol m(-2) s(-1)), including selected combinations. Using a…

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